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美国Product Name | Human IL-2 Protein |
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Catalog Number | orb613659 |
Reactivity | Human |
Conjugation | Unconjugated |
Endotoxins | 1.0 EU per μg |
Target | IL-2 |
Alternative Names | (click to expand) |
Form/Appearance | Powder |
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Preservatives | PBS, pH7.4 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Tag | C-10×His |
Note | For research use only. |
Purity | 90% |
MW | 17.3 kDa |
Source | Human IL-2, His Tag (orb613659) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). |
Biological Origin | Human |
Expression Region | Ala 21 - Thr 153 |
Activity | Loaded Biotinylated Human IL-2RB&IL-2RA&IL-2RG, Fc,&Fc, on SA Biosensor, can bind Human IL-2, His Tag with an affinity constant of 2.41 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
NCBI | NP_000577.2 |
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
Application Notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.3 kDa. The protein migrates as 18 kDa and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
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