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美国Human PLAU / uPA Protein(activated by trypsin)
Catalog Number: orb613660
Product Overview
Product Name | Human PLAU / uPA Protein(activated by trypsin) |
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Catalog Number | orb613660 |
Reactivity | Human |
Conjugation | Unconjugated |
Endotoxins | 1.0 EU per μg |
Target | PLAU |
Alternative Names | (click to expand) |
Product Properties
Form/Appearance | Powder |
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Preservatives | 25 mM HEPES, 150 mM NaCl, pH7.5 |
Storage | -20℃ |
Tag | C-6×His |
Note | For research use only. |
Protein Sequence | NP_002649.1 |
Purity | 90% |
MW | 15.3 kDa, 17.8 kDa and 29.2 kDa |
Source | Human PLAU, His Tag (activated by trypsin) (orb613660) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # P00749-1). |
Biological Origin | Human |
Expression Region | Ser 21 - Leu 431 |
NCBI | NP_002649.1 |
Product Description
Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
Application Notes
Application Notes | This protein carries a polyhistidine tag at the C-terminus. The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa. The protein migrates as 17 kDa (N-terminal fragment), 32-35 kDa (chain B) and 45-50 kDa (long chain A & chain B) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
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