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Product Overview
Product Name AKT antibody
Catalog Number orb29949
Species/HostRabbit
ReactivityHuman, Mouse
Predicted Reactivity Bovine, Rat
ConjugationUnconjugated
Tested applicationsELISA, FACS, ICC, IF, IHC-P, WB
Immunogen This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the C-terminal region of human AKT1.
Target AKT
Alternative Names
Product Properties
Form/Appearance PBS with 0.09% (W/V) sodium azide
Storage Store at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Peptide Affinity purified
MW 55.686 kDa
Uniprot ID P31749 (Human)
Entrez 207
Product Description

Rabbit polyclonal antibody to AKT

Application Notes
Dilution Range WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50, FACS: 1:10-50
Validation Images
Western blot analysis of mouse cerebellum tissue lysates (35ug/lane) using AKT1 antibody (C-term T450)
Western blot analysis of mouse cerebellum tissue lysates (35ug/lane) using AKT1 antibody (C-term T450)
Immunohistochemical analysis of formalin-fixed paraffin embedded human breast carcinoma tissue using AKT1 antibody (C-term T450)
Immunohistochemical analysis of formalin-fixed paraffin embedded human breast carcinoma tissue using AKT1 antibody (C-term T450)
Confocal immunofluoroscence analysis of MCF-7 cell using AKT1 antibody (C-term T450)
Confocal immunofluoroscence analysis of MCF-7 cell using AKT1 antibody (C-term T450)
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram) using AKT1 antibody (C-term T450)
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram) using AKT1 antibody (C-term T450)
References

Emodin protects hyperglycemia-induced injury in PC-12 cells by up-regulation of miR-9.

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