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Goat Anti-Rabbit IgG (H&L) Antibody (biotin)
Catalog Number: orb1236938
Product Overview
| Product Name | Goat Anti-Rabbit IgG (H&L) Antibody (biotin) |
|---|---|
| Catalog Number | orb1236938 |
| Species/Host | Goat |
| Reactivity | Rabbit |
| Conjugation | Biotin |
| Tested applications | ELISA |
| Immunogen | Rabbit IgG - whole molecule |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin |
| Storage | Biotin Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration. For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. |
| Note | For research use only. |
| Isotype | IgG |
Product Description
Goat Anti-Rabbit IgG (H&L) Antibody (biotin)
Application Notes
| Dilution Range | Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
|---|---|
| Application Notes | Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user). |
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