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Goat Anti-Chicken IgY (Fc) Antibody (HRP)
Catalog Number: orb1236934
Product Overview
| Product Name | Goat Anti-Chicken IgY (Fc) Antibody (HRP) |
|---|---|
| Catalog Number | orb1236934 |
| Species/Host | Goat |
| Reactivity | Gallus |
| Conjugation | HRP |
| Tested applications | ELISA, WB |
| Immunogen | Chicken IgY Fc fragment |
Product Properties
| Form/Appearance | Liquid |
|---|---|
| Preservatives | HEPES |
| Storage | 4 °C, avoid frequent freeze-thaw |
| Note | For research use only. |
| Isotype | IgY |
Product Description
Goat Anti-Chicken IgY (Fc) Antibody (HRP)
Application Notes
| Dilution Range | The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 μg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 – 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system. Optimal conjugate working dilutions should be determined for your particular assay conditions. |
|---|---|
| Application Notes | The affinity purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 μg/mL chicken IgY, conjugated antibody diluted to 1:100,000 produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Recommended Western Blot dilution: 1:2,000 – 1:4,000 when used for colorimetric assay, and 1:10,000 or up for ECL detection system. Optimal conjugate working dilutions should be determined for your particular assay conditions. |
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