TNF alpha antibody (APC)

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Catalog Number: orb1294064

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Product Overview

Product Name	TNF alpha antibody (APC)
Catalog Number	orb1294064
Species/Host	Mouse
Reactivity	Human
Conjugation	APC
Tested applications	FC, IF
Immunogen	E. coli-expressed, recombinant human TNF-α
Target	TNF
Alternative Names	(click to expand) TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Product Properties

Form/Appearance	Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Preservatives	Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Storage	Shipped and store at 4°C for one year. Do not freeze.
Note	For research use only.
Clonality	Monoclonal
Source	Mouse
Clone ID	MAb11
Uniprot ID	P01375
Entrez	7124

Product Description

Mouse monoclonal antibody against TNF alpha conjugated to APC

Application Notes

Dilution Range	10 μl / assay
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Dilution Range

10 μl / assay

Application Notes

1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

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