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Product Overview
| Product Name | Human ADM(Adrenomedullin) ELISA Kit |
|---|---|
| Catalog Number | orb1973174 |
| Reactivity | Human |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.91 pg/mL |
| Alternative Names | (click to expand) |
Product Properties
| Note | For research use only. |
|---|---|
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Product Description
Human ADM(Adrenomedullin) ELISA Kit
Application Notes
| Application Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADM in the samples is then determined by comparing the OD of the samples to the standard curve. |
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