Human HAS3(Hyaluronan Synthase 3) ELISA Kit

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Catalog Number: orb1994907

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Product Overview

Product Name	Human HAS3(Hyaluronan Synthase 3) ELISA Kit
Catalog Number	orb1994907
Reactivity	Human
Range	78.13-5000 pg/mL
Sensitivity	26.3 pg/mL
Alternative Names	(click to expand) Hyaluronic acid synthase 3; HA synthase 3

Product Properties

Note	For research use only.
Sample Types	Serum, plasma, tissue homogenates and other biological fluids

Product Description

Human HAS3(Hyaluronan Synthase 3) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HAS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HAS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HAS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HAS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HAS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HAS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HAS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HAS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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