Chicken GPX3(Glutathione Peroxidase 3) ELISA Kit

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Catalog Number: orb1994905

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Product Overview

Product Name	Chicken GPX3(Glutathione Peroxidase 3) ELISA Kit
Catalog Number	orb1994905
Reactivity	Gallus
Range	78.13-5000 pg/mL
Sensitivity	21.2 pg/mL
Alternative Names	(click to expand) GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase

Product Properties

Note	For research use only.
Sample Types	Serum, plasma and other biological fluids

Product Description

Chicken GPX3(Glutathione Peroxidase 3) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GPX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken GPX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken GPX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GPX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken GPX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GPX3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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