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Product Overview
| Product Name | Mouse E2(Estradiol) ELISA Kit |
|---|---|
| Catalog Number | orb1994898 |
| Reactivity | Mouse |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 4.45 pg/mL |
| Alternative Names | (click to expand) |
Product Properties
| Note | For research use only. |
|---|---|
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Product Description
Mouse E2(Estradiol) ELISA Kit
Application Notes
| Application Notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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