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美国Product Overview
Product Name | Human OXB(Orexin B) ELISA Kit |
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Catalog Number | orb1994893 |
Reactivity | Human |
Range | 0.4-25 ng/mL |
Sensitivity | 0.11 ng/mL |
Alternative Names | (click to expand) |
Product Properties
Note | For research use only. |
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Sample Types | serum, plasma and other biological fluids |
Product Description
Human OXB(Orexin B) ELISA Kit
Application Notes
Application Notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OXB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OXB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OXB in the samples is then determined by comparing the OD of the samples to the standard curve. |
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