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Product Overview
Product Name Human OXB(Orexin B) ELISA Kit
Catalog Number orb1994893
ReactivityHuman
Range 0.4-25 ng/mL
Sensitivity 0.11 ng/mL
Alternative Names
Product Properties
Note For research use only.
Sample Types serum, plasma and other biological fluids
Product Description

Human OXB(Orexin B) ELISA Kit

Application Notes
Application Notes This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OXB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OXB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OXB in the samples is then determined by comparing the OD of the samples to the standard curve.
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