Human SPAM1(Sperm Adhesion Molecule 1) ELISA Kit

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Catalog Number: orb1994891

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Product Overview

Product Name	Human SPAM1(Sperm Adhesion Molecule 1) ELISA Kit
Catalog Number	orb1994891
Reactivity	Human
Range	15.63-1000 pg/mL
Sensitivity	4.22 pg/mL
Alternative Names	(click to expand) HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n

Product Properties

Note	For research use only.
Sample Types	serum, plasma and other biological fluids

Product Description

Human SPAM1(Sperm Adhesion Molecule 1) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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