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Mouse ST2(Suppression of Tumorigenicity 2) ELISA Kit
Catalog Number: orb1994878
Product Overview
| Product Name | Mouse ST2(Suppression of Tumorigenicity 2) ELISA Kit |
|---|---|
| Catalog Number | orb1994878 |
| Reactivity | Mouse |
| Range | 0.16-10 ng/mL |
| Sensitivity | 0.049 ng/mL |
| Alternative Names | (click to expand) |
Product Properties
| Note | For research use only. |
|---|---|
| Sample Types | serum, plasma, tissue homogenates, cell lysates and other biological fluids |
Product Description
Mouse ST2(Suppression of Tumorigenicity 2) ELISA Kit
Application Notes
| Application Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ST2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ST2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ST2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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