Rat CA19-9(Carbohydrate Antigen 19-9) ELISA Kit

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Catalog Number: orb1994874

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Product Overview

Product Name	Rat CA19-9(Carbohydrate Antigen 19-9) ELISA Kit
Catalog Number	orb1994874
Reactivity	Rat
Range	0.79-50 U/mL
Sensitivity	0.31 U/mL
Alternative Names	(click to expand) sialylated Lewis(a)Antigen; Cancer Antigen 19-9

Product Properties

Note	For research use only.
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Description

Rat CA19-9(Carbohydrate Antigen 19-9) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

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