Dog vWF(Von Willebrand Factor) ELISA Kit

Skip to the end of the images gallery

Catalog Number: orb1994873

Be the first to review this product

References (0)

Images (0)

Datasheet

Product Overview

Product Name	Dog vWF(Von Willebrand Factor) ELISA Kit
Catalog Number	orb1994873
Reactivity	Canine
Range	0.63-40 ng/mL
Sensitivity	0.18 ng/mL
Alternative Names	(click to expand) F8VWF; VWD; von Willebrand antigen 2

Product Properties

Note	For research use only.
Sample Types	Plasma.

Product Description

Dog vWF(Von Willebrand Factor) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog vWF in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog vWF in the samples is then determined by comparing the OD of the samples to the standard curve.

Reviews

Write Your Own Review