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美国Product Overview
Product Name | Human ACV-A(Activin A) ELISA Kit |
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Catalog Number | orb1994864 |
Reactivity | Human |
Range | 15.63-1000 pg/mL |
Sensitivity | 4.63 pg/mL |
Alternative Names | (click to expand) |
Product Properties
Note | For research use only. |
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Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Product Description
Human ACV-A(Activin A) ELISA Kit
Application Notes
Application Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve. |
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