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Product Overview
Product Name Rat ACV-A(Activin A) ELISA Kit
Catalog Number orb1994863
ReactivityRat
Range 15.63-1000 pg/mL
Sensitivity 4.66 pg/mL
Alternative Names
Product Properties
Note For research use only.
Sample Types Serum, plasma and other biological fluids
Product Description

Rat ACV-A(Activin A) ELISA Kit

Application Notes
Application Notes The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.
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