Sheep FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

Skip to the end of the images gallery

Catalog Number: orb1994861

Be the first to review this product

References (0)

Images (0)

Datasheet

Product Overview

Product Name	Sheep FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit
Catalog Number	orb1994861
Reactivity	Sheep
Range	15.63-1000 pg/mL
Sensitivity	0.67 pg/mL
Alternative Names	(click to expand) ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

Product Properties

Note	For research use only.
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Product Description

Sheep FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep FGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep FGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep FGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep FGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep FGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep FGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep FGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep FGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Reviews

Write Your Own Review