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中国Human FOXO3(Forkhead Box Protein O3) ELISA Kit
Catalog Number: orb1994858
Product Overview
Product Name | Human FOXO3(Forkhead Box Protein O3) ELISA Kit |
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Catalog Number | orb1994858 |
Reactivity | Human |
Range | 15.63-1000 pg/mL |
Sensitivity | 6.61 pg/mL |
Alternative Names | (click to expand) |
Product Properties
Note | For research use only. |
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Sample Types | serum, plasma, other biological fluids |
Product Description
Human FOXO3(Forkhead Box Protein O3) ELISA Kit
Application Notes
Application Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FOXO3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FOXO3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FOXO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FOXO3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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