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美国Product Overview
Product Name | Sheep Testo(Testosterone) ELISA Kit |
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Catalog Number | orb1994857 |
Reactivity | Sheep |
Range | 0.32-20 ng/mL |
Sensitivity | 0.17 ng/mL |
Alternative Names | (click to expand) |
Product Properties
Note | For research use only. |
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Sample Types | serum, plasma, other biological fluids |
Product Description
Sheep Testo(Testosterone) ELISA Kit
Application Notes
Application Notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sheep Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep Testo in the samples is then determined by comparing the OD of the samples to the standard curve. |
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