Chicken CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

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Catalog Number: orb1994853

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Product Overview

Product Name	Chicken CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
Catalog Number	orb1994853
Reactivity	Gallus
Range	0.16-10 ng/mL
Sensitivity	0.054 ng/mL
Alternative Names	(click to expand) CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule

Product Properties

Note	For research use only.
Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids

Product Description

Chicken CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit

Application Notes

Application Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

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