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Product Overview
| Product Name | CD133 antibody (FITC) |
|---|---|
| Catalog Number | orb15325 |
| Species/Host | Rabbit |
| Conjugation | FITC |
| Tested applications | ICC, IF, IHC-P |
| Target | CD133 (FITC) |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS, 2 mM Sodium Azide, 30% glycerol |
|---|---|
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Immunogen affinity purified |
| MW | 95 kDa |
| Uniprot ID | O54990, O43490 |
| NCBI | NM_001145847.1, NP_001139319.1 |
| Entrez | 8842 |
Product Description
CD133 antibody (FITC)
Application Notes
| Dilution Range | IF/ICC: 1:50-100 |
|---|
Validation Images 
Immunofluorescence image of mouse uterus tissue using CD133 antibody (FITC) (dilution at 5 ug/ml)
Human prostate circulating tumor cells (CTCs) enumerated using the Veridex CellSearch system using a 1:50 dilution of CD133-FITC (orb15325) in the open channel. Screen shot showing cytokeratin/DAPI/CD133 (UDA channel) positive, CD45 negative CTC.
IF image of mouse skin tissue using anti-CD133 (FITC) (1:100)
Control
PC3 prostate cancer cells fixed and stained with normal rabbit IgG (control), and a 1:50 dilution of CD133-FITC (orb15325) (top image) in TBS for 1hr.
Immunofluorescence analysis of mouse skin tissue using anti-CD133 (FITC)(1:100)
Immunofluorescence analysis of mouse uterus tissue using anti-CD133 (dilution of primary antibody - 5 ug/ml)
IF analysis of mouse brain tissue using CD133 antibody (FITC) (1:100)
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