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Product Overview
| Product Name | CD133 antibody |
|---|---|
| Catalog Number | orb99113 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, ICC, IF, IHC-P, WB |
| Immunogen | KLH conjugated synthetic peptide derived from human CD133. Please contact us for the exact immunogen sequence. The peptide is available as orb374954. |
| Target | CD133. Human;Mouse;Rat. No cross reactivity with other proteins. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | 10 mM PBS, 0.02% sodium azide, 10 mg/ml rAlbumin |
|---|---|
| Concentration | - 100 μg (in 100 μl): 1 mg/ml |
| Storage | After reconstitution store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| MW | 95 kDa |
| Uniprot ID | |
| NCBI | 1, 001145847, 001139319, 11 |
| Entrez | 8842 |
Product Description
CD133 antibody
Application Notes
| Dilution Range | IHC-P:1:200, WB: 1:500, IF/ICC: 1:200 |
|---|
Validation Images 
![Western Blot on human cells using CD133 antibody (Protocol steps under Review A) [Reviews]](https://biorbyt.com.cn/pub/media/catalog/product/c/d/cd133-antibody-7ed_2.jpg)
![Western blot analysis of human cancer cell lysates using CD133 antibody [Reviews]](https://biorbyt.com.cn/pub/media/catalog/product/c/d/cd133-antibody-608_1.jpg)
IF analysis of mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:200)
IHC-P image of human endometrial cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)
Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-CD206 (primary antibody at 1:200)
Western blot analysis of Mouse large intestine (Lane 1), Mouse spinal cord (Lane 2) using CD133 antibody (Dilution of primary antibody 1:200)
IHC-P image of human breast cancer tissue using anti-CD206 (dilution of primary antibody at 1:100)
Immunohistochemical staining of human mammary fibroma tissue using anti-CD206 (dilution of primary antibody - 1:100)
Western blot analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)
Western blot analysis of human muscle (Lane1), Rat muscle (Lane2), Rat bladder (Lane3) using CD133 antibody (Dilution at: 1:500)
IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)
IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody diluted to 1:100)
IHC-P analysis of human lung cancer tissue using CD133 antibody (Primary antibody diluted to 1:100)
Immunohistochemical analysis of formalin-fixed and paraffin embedded human colonic adenocarcinoma tissue using CD133 antibody (Dilution of primary antibody 1:100)
IHC-P analysis of human muscle tissue using CD133 antibody (Primary antibody at 1:100)
IHC-P analysis of human colonic adenocarcinoma tissue using CD133 antibody (Primary antibody at 1:100)
Immunohistochemical analysis of formalin-fixed and paraffin embedded human muscle tissue using CD133 antibody (Primary antibody diluted to 1:200)
IHC-P analysis of human lung cancer tissue using CD133 antibody (Dilution of primary antibody 1:100)
Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse skin tissue using CD133 antibody (Dilution of primary antibody 1:100)
Immunofluorescent analysis of mouse brain tissue using CD133 antibody (Primary antibody diluted to 1:200)
IHC-P analysis of mouse brain tissue using CD133 antibody (Dilution of primary antibody 1:100)
IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody at 1:100)
Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-CD206 (primary antibody at 1:200)
IHC-P analysis of mouse liver tissue using CD133 antibody (Primary antibody at 1:100)
IHC-P analysis of mouse liver tissue using CD133 antibody (Dilution of primary antibody 1:100)
IF analysis analysis of mouse lung tissue using CD133 antibody (Dilution of primary antibody 1:200)
Western Blot on human cells using CD133 antibody (Protocol steps under Review A) [Reviews]
WB analysis of rat small intestine tissue using CD133 antibody (dilution at 1:200-1:1000 based on 1mg/mL)
Western blot analysis of human cancer cell lysates using CD133 antibody [Reviews]
IHC-P analysis of mouse heart tissue using CD133 antibody (Primary antibody diluted to 1:100)
Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue using CD133 antibody
References
- Liao, C. H. et al. Restoration of erectile function with intracavernous injections of endothelial progenitor cells after bilateral cavernous nerve injury in rats Andrology, 3, 924-932 (2015)
- Jensen, Todd et al. Descriptive analysis of tumor cells with stem like phenotypes in metastatic and benign adrenal tumors J Pediatr Surg, 50, 1493-1501 (2015)
- Sun, Zhonghai et al. Tumour stem cell markers CD133 and CD44 are useful prognostic factors after surgical resection of pancreatic neuroendocrine tumours Oncol Lett, 20, 341 (2020)
- Nel, Ivonne et al. Circulating Tumor Cell Composition in Renal Cell Carcinoma PLoS One, 11, e0153018 (2016)
- Tu, Zhenbo et al. CXCR4 is involved in CD133-induced EMT in non-small cell lung cancer Int J Oncol, 50, 505-514 (2017)
- Nadal, Rosa et al. CD133 expression in circulating tumor cells from breast cancer patients: potential role in resistance to chemotherapy Int J Cancer, 133, 2398-2407 (2013)
- Zhu, Yifan et al. Influence of interferon-α on the expression of the cancer stem cell markers in pancreatic carcinoma cells Exp Cell Res, 324, 146-156 (2014)
- Yang, Meng-Yin et al. An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior J Biomed Mater Res B Appl Biomater, 103, 618-628 (2015)
- Coni, Pierpaolo et al. Exploring cell surface markers and cell-cell interactions of human breast milk stem cells J Public Health Res, 12, (2023)
- Zou, Yuhong et al. Nrf2 is involved in maintaining hepatocyte identity during liver regeneration PLoS One, 9, e107423 (2014)
- Nowacki, Maciej et al. Nanovehicles as a novel target strategy for hyperthermic intraperitoneal chemotherapy: a multidisciplinary study of peritoneal carcinomatosis Oncotarget, 6, 22776-22798 (2015)
- Hirashima, Kanji et al. Cell biological profiling of reprogrammed cancer stem cell-like colon cancer cells maintained in culture Cell Tissue Res, 375, 697-707 (2019)
- Kase, Marju et al. Impact of CD133 positive stem cell proportion on survival in patients with glioblastoma multiforme Radiol Oncol, 47, 405-410 (2013)
- Feitosa, Neli Patrícia Pereira et al. Cancerous and non-neoplastic stem cells in the stomach similarly express CD44 and CD133 Acta Histochem, 123, 151787 (2021)
- Chen, Jintao et al. Proteomic Distributions in CD34+ Microvascular Niche Patterns of Glioblastoma J Histochem Cytochem, (2021)
- Winardi, Daniel et al. Correlation of altered expression of the autophagy marker LC3B with poor prognosis in astrocytoma Biomed Res Int, 2014, 723176 (2014)
- Feng, Long et al. Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer Mol Med Rep, 12, 3531-3536 (2015)
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