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Product Overview
| Product Name | CD163 antibody |
|---|---|
| Catalog Number | orb13303 |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Tested applications | ICC, IF, IHC-P, WB |
| Immunogen | KLH conjugated synthetic peptide derived from human CD163. Please contact us for the exact immunogen sequence. The peptide is available as orb374759. |
| Target | CD163 |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
|---|---|
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml - 200 μg (in 400 μl): 0.5 mg/ml |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| MW | 125 kDa |
| Uniprot ID | Q2VLH6, Q86VB7, D3Z9U2 |
| NCBI | 41, 004235, 51, 4, 004244, 5 |
| Entrez | 9332 |
Product Description
CD163 antibody
Application Notes
| Dilution Range | IHC-P: 1: 100-600, WB: 1:500, ICC: 1:100 |
|---|
Validation Images 
Immunohistochemical staining of paraffin embedded human lung cancer tissue using CD163 antibody (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of guinea pig colon tissue using anti-CD163 (2.5 ug/ml)
IHC-P image of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of mouse stomach tissue using anti-CD163 (2.5 ug/ml)
Immunohistochemical staining of rat lymph tissue using CD163 antibody (2.5 ug/ml)
IHC-P image of rat lymph tissue using anti-CD163 (2.5 ug/ml)
Western blot analysis of guniea pig lymph (lane 1), rat brain (lane 2), mouse stomach (lane3), rat rectum (lane4), human gallbladder (lane 5), human lung cancer (lane 6) tissue using anti-CD163 (0.5 ug/ml)
Immunohistochemical staining of mouse skin tissue using CD163 antibody (dilution of primary antibody - 1:200)
Immunofluorescent analysis of mouse lung tissue using CD163 antibody (Primary antibody diluted to 1:100)
Immunohistochemical staining of mouse liver tissue using anti-CD163 (dilution of primary antibody - 1:200)
Immunofluorescent image of mouse liver tissue using CD163 antibody (Primary antibody at 1:100)
IF analysis of mouse skin tissue using CD163 antibody (Dilution of primary antibody 1:100)
Immunohistochemical staining of paraffin embedded mouse liver tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of human colon cancer tissue using CD163 antibody. (Dilution of primary antibody 1:200)
Immunohistochemical staining of paraffin embedded human colon cancer tissue (orb13303 at 1:200) using CD163 antibody. (Dilution of primary antibody 1:200)
Western blot analysis of human cell lysates using CD163 antibody (please check application notes for details)
IHC-P of human lung adenocarcinoma tissue using CD163 antibody (Dilution of primary antibody 1:200)
IHC-P image of human thyroid carcinoma tissue using CD163 antibody (dilution of primary antibody at 1:500)
WB analysis of human breast cancel 25 (Lane1), breast cancel 24 (Lane2) using CD163 antibody (dilution at 1:100)
Immunohistochemical staining of pig large intestines tissue using anti-CD163 (dilution of primary antibody - 1:200)
IHC-P staining of pig lung tissue using anti-CD163 (dilution at 1:200)
Immunofluorescence image of rat skin tissue using anti-CD163 (dilution at 1:100)
WB analysis of human breast cancer (Lane 1), breast cancer (Lane 2), breast cancer (Lane 3) using anti-CD163 (dilution at 1:500)
Immunohistochemical staining of paraffin embedded mouse skin tissue using CD163 antibody (primary antibody at 1:200)
Additional Documents
References
- Koronyo-Hamaoui, Maya et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease Brain, 143, 336-358 (2020)
- Gibson, D. L. et al. Maternal exposure to fish oil primes offspring to harbor intestinal pathobionts associated with altered immune cell balance Gut Microbes, 6, 24-32 (2015)
- Koronyo, Yosef et al. Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's disease Brain, 138, 2399-2422 (2015)
- Heindryckx, Femke et al. Inhibition of the placental growth factor decreases burden of cholangiocarcinoma and hepatocellular carcinoma in a transgenic mouse model Eur J Gastroenterol Hepatol, 24, 1020-1032 (2012)
- de Vries, M. R. et al. Blockade of vascular endothelial growth factor receptor 2 inhibits intraplaque haemorrhage by normalization of plaque neovessels J Intern Med, 285, 59-74 (2019)
- Mertens, Barbara et al. Investigation of tumor-tumor interactions in a double human cervical carcinoma xenograft model in nude mice Oncotarget, 9, 21978-22000 (2018)
- Chen-Roetling, Jing et al. Haptoglobin increases the vulnerability of CD163-expressing neurons to hemoglobin J Neurochem, 139, 586-595 (2016)
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