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Product Overview
| Product Name | Interleukin-18 IL18 Antibody |
|---|---|
| Catalog Number | orb610896 |
| Species/Host | Rabbit |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | IHC, WB |
| Immunogen | E. coli-derived human IL-18 recombinant protein (Position: Y37-D193). |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| MW | 22326 MW |
| Uniprot ID | Q14116 |
Product Description
Interleukin-18 IL18 Antibody
Application Notes
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Western blot, 0.1-0.5μg/ml, Human |
|---|---|
| Application Notes | WB: The detection limit for IL-18 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Validation Images 
Anti-IL-18 antibody,IHC(P):Human Tonsil Tissue
Anti-IL-18 antibody, PA2037, Western blotting. All lanes:Anti IL-18 at 0.5ug/ml, Lane 1:HELA cell Lysate at 40ug, Lane 2:Placenta Tissue Lysate at 50ug,Predicted bind size:22KD,Observed bind size:22KD
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