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Product Overview
| Product Name | MVP Antibody (monoclonal, 8B12) |
|---|---|
| Catalog Number | orb610911 |
| Species/Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | FC, ICC, IF, IHC, WB |
| Immunogen | E.coli-derived human MVP recombinant protein (Position: A2-H259). |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | Mouse IgG2a |
| Clonality | Monoclonal |
| MW | 100-110 kDa |
| Clone ID | 8B12 |
| Uniprot ID | Q14764 |
Product Description
MVP Antibody (monoclonal, 8B12)
Application Notes
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
|---|---|
| Application Notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Validation Images 
WB analysis using anti-MVP antibody.Lane 1:A549 tissue, Lane 2:U2OS cell, Lane 3:HepG2 cell, Lane 4:THP-1 cell, Lane 5:HeLa cell, Lane 6:SW620 cell.Lane 7:rat RH35 cell.Lane 8:mouse RAW246.7 cell.
IHC analysis of MVP using anti-MVP antibody.
IHC analysis of MVP using anti-MVP antibody.
IHC analysis of MVP using anti-MVP antibody.
IHC analysis of MVP using anti-MVP antibody.
IHC analysis of MVP using anti-MVP antibody.
IHC analysis of MVP using anti-MVP antibody.
Flow Cytometry analysis of A549 cells using anti-MVP antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-MVP antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.
IF analysis of MVP using anti-MVP antibody.
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