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Product Overview
| Product Name | Nestin antibody |
|---|---|
| Catalog Number | orb11109 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, IHC-P, WB |
| Immunogen | KLH conjugated synthetic peptide derived from human Nestin. Please contact us for the exact immunogen sequence. The peptide is available as orb374738. |
| Target | Nestin. Recognized endogenous levels of total Nestin |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
|---|---|
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml - 200 μg (in 400 μl): 0.5 mg/ml |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| MW | 177 kDa |
| Uniprot ID | P48681, P21263, Q6P5H2 |
| NCBI | 006617, 006608, 1 |
| Entrez | 10763 |
Product Description
Rabbit polyclonal to Nestin
Application Notes
| Dilution Range | WB: 1:50-500, IHC-P: 1:100-500 (based on 0.5 mg/ml) |
|---|
Validation Images 
Immunohistochemical staining of rat brain tissue using Nestin antibody (5 ug/ml)
IHC-P staining of rat pancreas tissue using anti-Nestin (2.5 ug/ml)
IHC-P image of rat brain tissue using anti-Nestin (5 ug/ml)
IHC-P image of rat kidney tissue using Nestin antibody (5 ug/ml)
IHC-P staining of rat kidney tissue using Nestin antibody (2.5 ug/ml)
IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)
IHC-P image of rat kidney tissue using Nestin antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat kidney tissue using Nestin antibody (5 ug/ml)
Western blot analysis of U251 cells using Nestin antibody (3 ug/ml)
Immunohistochemical staining of mouse bone marrow tissue using anti-Nestin (2.5 ug/ml)
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