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Product Overview
Product Name tdTomato antibody
Catalog Number orb182397
Species/HostGoat
ReactivityOther
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-Fr, IHC-P, WB
Immunogen Purified recombinant peptide produced in E. coli.
Target tdTomato
Alternative Names
Product Properties
Form/Appearance ​Polyclonal antibody supplied in PBS, 20% glycerol and 0.05% sodium azide
Concentration 1mg/ml
Preservatives PBS, 20% glycerol and 0.05% sodium azide
Storage For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity This antibody is epitope-affinity purified from goat antiserum
MW 55 kDa
Product Description

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation

Application Notes
Dilution Range WB 1:500-1:2,000, IHC (F) 1:50-1:500, IHC (P) 1:50-1:500, IF 1:50-1:500
Application Notes In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein)
Validation Images
Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.
Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.
References

  1. Yang, Yang et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency Cell, 169, 243-257.e25 (2017)
  2. Mao, Xiying et al. Single-Cell RNA Sequencing of hESC-Derived 3D Retinal Organoids Reveals Novel Genes Regulating RPC Commitment in Early Human Retinogenesis Stem Cell Reports, (2019)
  3. Li Li # 1 2 3, Yu He # 1 2 3, Kai Liu 4, Lin Liu 1 2 3, Shan Shan 1 2 3, Helin Liu 1 2 3, Jiangbo Ren 1 2 3, Shujie Sun 1 2 3, Min Wang 1 2 3, Jidong Jia 5 6 7, Ping Wang GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice Cell Death Dis, 15, 114 (2024)
  4. Pomaville, Matthew B. et al. Follicle-innervating A[delta]-low threshold mechanoreceptive neurons form receptive fields through homotypic competition Neural Dev, 18, 2 (2023)
  5. Fang, Yinshan et al. Epithelial Wntless regulates postnatal alveologenesis Development, 149, dev199505 (2022)
  6. Li, Hui et al. Identification of novel B-1 transitional progenitors by B-1 lymphocyte fate-mapping transgenic mouse model Bhlhe41 dTomato-Cre Front Immunol, 13, 946202 (2022)
  7. Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells Elife, 11, e78217 (2022)
  8. Miller, Daniel S. et al. Neuronal Dystroglycan regulates postnatal development of CCK/cannabinoid receptor-1 interneurons Neural Dev, 16, 4 (2021)
  9. SOX9 Modulates the Transformation of Gastric Stem Cells Through Biased Symmetric Cell Division Gastroenterology, S0016-5085(23)00109-9 (2023)
  10. Therapeutic resistance and susceptibility is shaped by cooperative multi-compartment tumor adaptation Cell Death Differ, 26, 2416-2429 (2019)
  11. Yartseva, Valeria et al. Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal Cell Rep, 30, 1491-1503.e6 (2020)
  12. Interleukin-9 protects from early podocyte injury and progressive glomerulosclerosis in Adriamycin-induced nephropathy Kidney Int, 98, 615-629 (2020)
  13. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis Cell, 178, 1478-1492.e20 (2019)
  14. Jiang, Ming et al. VEGF receptor 2 (KDR) protects airways from mucus metaplasia through a Sox9-dependent pathway Dev Cell, 56, 1646-1660.e5 (2021)
  15. Hu, Yanyan et al. Induction of mouse totipotent stem cells by a defined chemical cocktail Nature, (2022)
  16. Zhang, Lianghui et al. Single-cell transcriptomic profiling of lung endothelial cells identifies dynamic inflammatory and regenerative subpopulations JCI Insight, 7, e158079 (2022)
  17. He, Jianbo et al. DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration NPJ Regen Med, 7, 21 (2022)
  18. Zhang, Junren et al. Tel2 regulates redifferentiation of bipotential progenitor cells via Hhex during zebrafish liver regeneration Cell Rep, 39, 110596 (2022)
  19. Gribben, Christopher et al. Ductal Ngn3-expressing progenitors contribute to adult β cell neogenesis in the pancreas Cell Stem Cell, (2021)
  20. Gu, Bin et al. Live imaging YAP signalling in mouse embryo development Open Biol, 12, 210335 (2022)

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