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mtTFA TFAM Antibody (monoclonal, 4D9)
Catalog Number: orb610917
Product Overview
| Product Name | mtTFA TFAM Antibody (monoclonal, 4D9) |
|---|---|
| Catalog Number | orb610917 |
| Species/Host | Mouse |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | FC, IHC, WB |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | Mouse IgG2b |
| Clonality | Monoclonal |
| MW | 40 kDa |
| Clone ID | 4D9 |
| Uniprot ID | Q00059 |
Product Description
mtTFA TFAM Antibody (monoclonal, 4D9)
Application Notes
| Dilution Range | Western blot, 1-2μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human |
|---|---|
| Application Notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Validation Images 
WB analysis using anti ZO-1 antibody.Lane 1:HEK293 tissue, Lane 2:K562 cell, Lane 3:Caco-2 cell, Lane 4:Raji cell, Lane 5:A549 cell,
IHC analysis of TFAM using anti-TFAM antibody.
IHC analysis of TFAM using anti-TFAM antibody.
Flow Cytometry analysis of CACO-2 cells using anti-FAM antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.
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