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Product Overview
| Product Name | TJP1 Antibody (monoclonal, 3E12) |
|---|---|
| Catalog Number | orb610914 |
| Species/Host | Mouse |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | FC, ICC, IF, IHC, WB |
| Immunogen | E.coli-derived human TJP1 recombinant protein (Position: H1178-F1527). Human TJP1 shares 82% amino acid (aa) sequence identity with mouse TJP1. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | Mouse IgG1 |
| Clonality | Monoclonal |
| MW | 220 kDa |
| Clone ID | 3E12 |
| Uniprot ID | Q07157 |
Product Description
TJP1 Antibody (monoclonal, 3E12)
Application Notes
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
|---|---|
| Application Notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Validation Images 
WB analysis using anti-TJP1 antibody.Lane 1:CACO-2 cell, Lane 2:U20S cell, Lane 3:K562 cell, Lane 4:PC-3 cell, Lane 5:T47D cell.
IHC analysis of TJP1 using anti-TJP1 antibody.
IHC analysis of TJP1 using anti-TJP1 antibody.
Flow Cytometry analysis of hela cells using anti-TJP1 antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.
IF analysis of TJP1 using anti-TJP1 antibody.
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