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Product Overview
| Product Name | VEGF antibody |
|---|---|
| Catalog Number | orb256347 |
| Species/Host | Mouse |
| Reactivity | Human, Porcine, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, IHC-P, WB |
| Immunogen | Human VEGFA recombinant protein. Protein available as (orb178366) was used as immunogen. |
| Target | VEGF |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
|---|---|
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml - 200 μg (in 400 μl): 0.5 mg/ml |
| Storage | After reconstitution store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Purity | Monoclonal antibodies are purified by peptide affinity chromatography |
| MW | 27 kDa |
| Clone ID | 4H4G2 |
| Uniprot ID | P15692, Q00731, P16612 |
| NCBI | 15692, 14 |
| Entrez | 5788 |
Product Description
Mouse monoclonal antibody to VEGF
Application Notes
| Dilution Range | IHC-P: 5 μg/ml, WB: 1μg/ml |
|---|
Validation Images 
Immunohistochemical staining of pig large intestine tissue using anti-VEGFA (5 ug/ml)
Immunohistochemical staining of paraffin embedded rat colon tissue using VEGFA antibody (5 ug/ml)
WB analysis of mouse brain (lane 1), mouse liver (lane 2), mouse kidney (lane 3), hela cells (lane 4) tissue using VEGFA antibody (1 ug/ml)
WB analysis of protein 100 ng (lane 1), protein 50 ng (lane 2), protein 20 ng (lane 3) tissue using VEGFA antibody (1 ug/ml)
References
- Bonacina, F. et al. Pentraxin 3 deficiency protects from the metabolic inflammation associated to diet-induced obesity Cardiovasc. Res., (2019)
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