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Product Overview
| Product Name | Desmoglein 2/DSG2 Antibody |
|---|---|
| Catalog Number | orb610895 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Hamster |
| Conjugation | Unconjugated |
| Tested applications | FC, ICC, IF, IHC, WB |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 2 (1104-1118aa STRVTKHSTVQHSYS), different from the related mouse and rat sequences by one amino acid. |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Lyophilized |
|---|---|
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| MW | 122294 MW |
| Uniprot ID | Q14126 |
Product Description
Desmoglein 2/DSG2 Antibody
Application Notes
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
|---|---|
| Application Notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Validation Images 
WB analysis using anti-Desmoglein 2 antibody.Lane 1:K562 cell, Lane 2:HeLa cell, Lane 3:HepG2 cell, Lane 4:A549 cell, Lane 5:Caco-2 cell, Lane 6:SW620 cell, Lane 7:rat liver tissue, Lane 8:mouse liver tissue,
IHC analysis of DSG2 using anti-DSG2 antibody.
IHC analysis of DSG2 using anti-DSG2 antibody.
IHC analysis of DSG2 using anti-DSG2 antibody.
IF analysis of DSG2 using anti-DSG2 antibody.
Flow Cytometry analysis of 293T cells using anti-DSG2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.
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